FastQCFastQC Report
Thu 28 Apr 2016
ERR1041502.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041502.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11770931
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT653830.5554615858337798No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT604700.5137231710898653No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT478720.4066968024874158No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271580.23072091748732537No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA213800.019.1662771
GTATCAA314200.013.0124122
AACGCAG378050.010.6630085
GAGTACG226000.010.6579641
AGTACGG226450.010.5795972
CGCGAAA3208.6765795E-1010.4062515
GTACTAG7200.010.2777791
GTACCGT3301.4806574E-910.0909096
GTATTAG25250.010.0376241
TATACTG20650.09.944315
TAGACAG18600.09.8467745
TAATACT27300.09.7582434
TTAACGG7600.09.73684235
GGACCGT6700.09.6641796
TATCAAC431350.09.632783
ATCAACG425400.09.5761644
CTAATAC25750.09.4834963
CTTACAC23650.09.46511653
ACCGTCC5500.09.4181828
TCAACGC433150.09.4048265
Produced by FastQC (version 0.11.5)