FastQCFastQC Report
Thu 28 Apr 2016
ERR1041501.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041501.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12997663
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT945030.7270768598939671No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT853650.6567719135355332No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT677610.5213321810236194No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT393430.302692876404012No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142480.10961970625026976No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133290.10254920442236423No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA306950.019.1900961
GTATCAA450850.013.0692022
CGCTATA3351.4551915E-1111.0447762
GTATTAG29800.010.9261751
CTAGTAC8450.010.727813
AACGCAG543350.010.6910837
GAGTACG355550.010.3908021
AGTACGG355500.010.3610412
TAGACAG19300.010.2564765
TCTATAC15350.010.1237783
TAGTACT15450.09.8187694
TATCAAC617700.09.6168853
TAATACT31800.09.5990564
ATCAACG616900.09.5303944
TAAGAGT25650.09.5204684
ATACCGA5850.09.487186
ACCGCGT5500.09.4181828
CTACGCT3953.4742698E-109.3670884
TCAACGC629250.09.3286465
TACGGGT147950.09.3281524
Produced by FastQC (version 0.11.5)