FastQCFastQC Report
Thu 28 Apr 2016
ERR1041499.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041499.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9431649
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT565170.5992271340886414No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT526150.5578557895867414No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT403450.4277618897819459No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT214940.2278922805545457No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA179150.019.2796541
GTATCAA263700.013.0769822
TTAGACT10900.010.8623854
AACGCAG325100.010.8006765
GTATTAG20800.010.7620191
TATACTG15100.010.4139085
GGACCGT5400.010.2777786
GAGTACG203050.010.1041611
AGTACGG202400.010.0360672
TCTGTCG7450.09.6845648
ATCAACG361300.09.6775542
TATCAAC365050.09.5375983
TCAACGC369900.09.512573
GTACTAG7200.09.5069451
ACGCAGA371800.09.4689356
CAACGCA373200.09.45819954
TACGGGT72750.09.4089364
TAAGACT19350.09.3695094
CGCAGAG378800.09.2841867
TAATACT24400.09.2499994
Produced by FastQC (version 0.11.5)