FastQCFastQC Report
Thu 28 Apr 2016
ERR1041498.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041498.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9730033
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT669800.6883840990056251No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT642210.660028593942076No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT471830.484921274162174No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254300.2613557425755904No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA231600.018.2124351
GTATCAA326550.012.9111922
TTAACGG7650.012.09150335
TATACTG16500.011.4363645
AACGCAG382000.010.9401835
TAACGGC8600.010.75581436
GTATTAG23900.010.2175731
ATTAGAG14300.010.0909093
GAGTACG217550.010.025971
TTATACG4251.8189894E-1210.01176535
AGTACGG218600.09.91010952
ATCAACG432000.09.7596074
ATACTGT21800.09.6743126
TATCAAC442950.09.6519923
ACGCAGA434000.09.6421656
TCAACGC441250.09.5592075
TAATACT25650.09.5204684
CGCAGAG439800.09.5150077
CAACGCA445500.09.4472496
TAGACAG16100.09.422365
Produced by FastQC (version 0.11.5)