FastQCFastQC Report
Thu 28 Apr 2016
ERR1041497.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041497.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7479012
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT543360.7265130741868043No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT523380.6997983156063929No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT387640.5183037545600943No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237440.3174750889556No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85180.11389204884281506No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80130.10713982007249086No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA219100.015.6967131
ATCTCGT5300.012.56603837
TACGCTA4750.012.46315831
CTACGCT4750.012.46315830
GACGCTA5050.012.08910826
ACGCTAT4900.012.08163332
GCGTATA2401.4895704E-811.5624999
TATACTG13850.011.4873655
TTAACGG4550.011.38461635
GTATCAA303900.011.2923662
CGCTATC5850.010.75213733
AACGCAG318150.010.5656155
GAGTACG193500.010.0100771
TTAGACT9100.09.9615394
AGTACGG192400.09.93269352
CGCGAAA2054.2276137E-59.9268315
GTATTAG18250.09.8328761
CGCTACG6400.09.82812528
GAACCGT4159.094947E-129.8072296
GTATAGA11650.09.5278971
Produced by FastQC (version 0.11.5)