FastQCFastQC Report
Thu 28 Apr 2016
ERR1041496.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041496.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10118336
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT466130.4606785147281134No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT390180.3856167654444367No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT325640.32183157388724787No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177420.17534503697050582No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA129650.021.3752421
GTATCAA193500.014.2932822
TATACTG16800.012.1130955
CGCGAAA2705.802576E-1011.64814915
TAGACAG17150.011.1107875
GTATTAG22000.011.0159081
AACGCAG253600.010.738177
ATCAACG265600.010.3714244
GGACCGT5550.010.3333336
TATCAAC272350.010.2570223
CGACTTA2351.6403137E-610.23404310
TCAACGC269900.010.1993335
CAACGCA272600.010.1050986
CTACACT18200.010.0631874
TCACGTA9600.010.02083425
GAGTACG153150.09.7724461
TAATACT24750.09.7171724
TACTCCG12000.09.71255
AGTACGG153300.09.7025432
TTAGACT13900.09.449644
Produced by FastQC (version 0.11.5)