FastQCFastQC Report
Thu 28 Apr 2016
ERR1041495.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041495.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9395708
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT476490.5071358113725969No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT418000.4448839831974344No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT338570.3603453832324291No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194030.20650918483205313No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA158750.018.7272431
GTATCAA231650.012.7619262
TAACGCC2851.1459633E-1011.684214
TATACTG17400.010.951155
AACGCAG271900.010.6618257
ATACTGT23150.010.0691156
TTAGACT11100.010.04
TAGGACC13450.09.76579954
ATCAACG305500.09.65274
GAGTACG158900.09.6516681
TAGACAG15950.09.6269595
AGTACGG158200.09.60082152
TAATACT25650.09.5925924
TATCAAC313350.09.5820963
CTACACT16100.09.5372684
TCAACGC311150.09.5071515
CAACGCA314750.09.4160446
GTATTAG21550.09.3573091
ACGCAGA311900.09.2470358
CTCTTAT40300.09.08933137
Produced by FastQC (version 0.11.5)