FastQCFastQC Report
Thu 28 Apr 2016
ERR1041494.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041494.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9227063
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT577650.6260388598192079No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT501770.5438025079052782No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT402300.4360000576564829No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237450.25734082448553786No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA209400.016.5121771
GTACTAG6850.012.6934311
TAGACCG1807.479284E-712.3333345
GTATCAA294400.011.7447362
GTATTAG25700.011.3735411
TAATACT26600.010.9191724
GTCGGGA9100.010.7747262
TTTCGCG1250.00970340610.3628
TTAACGG8400.010.13095335
AACGCAG339700.010.069625
AGTACGG217000.09.9320272
GAGTACG218500.09.9231121
TACACAG22650.09.8830025
AATACTG23150.09.8293735
TAGACTA10700.09.6822425
TATACTG16900.09.6331375
CTAATAC28400.09.4454223
CGATCGC8250.09.41818219
TACCGTC4158.913048E-119.3614457
GTATTAA15700.09.3089171
Produced by FastQC (version 0.11.5)