FastQCFastQC Report
Thu 28 Apr 2016
ERR1041493.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041493.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5776272
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT285970.4950771016323331No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT280220.48512258425503507No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT208610.3611498904483722No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124130.21489638992069626No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA119600.014.5091981
TAGACCG1000.001834033212.9500015
TCTAGAC5250.012.6857143
TTAACGG3950.012.6455735
CTACGCT3051.8189894E-1212.13114730
GTACTAG3850.012.0129861
GTATTAG14050.011.4555161
GTATAGG10350.011.4396131
TATACTG9600.011.1770845
GACGCTA3650.011.15068526
TAGGACG2851.4224497E-911.0350884
TCTACGC2401.8462924E-710.7916673
CGCTATC3605.456968E-1210.79166633
GTATCAA165900.010.4710672
TAATACT15050.010.4485054
GGGGTTA7800.010.4358976
TAGACAG8400.010.3511915
TAGAACC8400.010.3511914
TAGGGGT8250.010.3151524
TTAGGAC7800.010.1987173
Produced by FastQC (version 0.11.5)