FastQCFastQC Report
Thu 28 Apr 2016
ERR1041492.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041492.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13728655
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT882870.6430855753895774No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT732110.5332714675982462No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT626430.45629378843011204No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT343390.2501264690532321No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA295100.017.8542861
TTAACGG12050.012.58921135
GTATCAA426150.012.3593812
TAACGGC13700.010.93795636
GTATACG6900.010.7246381
AACGCAG487500.010.5763077
GTATTAG38450.010.4408321
CTAGTAC8500.010.0117643
ATACCGA6550.09.8854966
CTACACT27700.09.817694
TAACCGG7950.09.77358422
TAGGACC23900.09.7531394
GAGTACG291750.09.7017991
AGTACGG290400.09.6449732
TATACTG19600.09.5331635
TATACCG6800.09.52205855
TAATACT39300.09.4618324
ATCAACG557450.09.4416534
TATCAAC572100.09.3486273
AACGGTA4950.09.34343418
Produced by FastQC (version 0.11.5)