FastQCFastQC Report
Thu 28 Apr 2016
ERR1041491.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041491.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10205452
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT616040.6036381338131814No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT482030.4723259685117327No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT429010.42037334554118716No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238550.2337476086311513No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107030.10487531566460752No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106570.10442457619711504No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA160350.023.00531
GTATCAA245600.015.0124182
TTAACGG11650.012.38626735
TAACGGC12000.011.71666636
GTATTAG30500.011.4639351
CGCGTAT1300.00100410711.38461512
AACGCAG330900.011.0306747
TCTATAC13800.010.9927533
ATCAACG341300.010.7866974
TATCAAC351000.010.69415953
TCAACGC347400.010.60794455
GAGTACG202900.010.5675221
AGTACGG202150.010.4877582
TAACCGG7600.010.46710622
CAACGCA351750.010.4557226
ATTAGAG20000.010.4524993
CCGTTTA3208.6765795E-1010.4062513
TAATACT32450.010.204934
TATACTG15150.010.0132025
ACCGCCT16650.09.88888912
Produced by FastQC (version 0.11.5)