FastQCFastQC Report
Thu 28 Apr 2016
ERR1041490.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041490.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10730148
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT543420.5064422224185539No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT431030.40169995791297564No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT374610.3491191361013846No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197550.1841074326281427No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA148700.022.5309351
GTATCAA225650.014.8065592
TTAACGG9850.011.45685235
TAATACT28600.011.4493014
AACGCAG295200.011.2867557
ATCAACG303750.010.9629634
TCAACGC307700.010.81020455
TATCAAC314850.010.7997463
CAACGCA311000.010.7014476
TAACGGC10550.010.69668236
CGCGAAA2801.2692908E-810.57142815
GTATTAG29700.010.5269361
GGGTAAG13750.010.361
GTATAGG18650.010.2171581
TAATACC13000.010.1038464
TAGGACC17500.010.0428574
ATTAGAG18750.09.9653333
GAGTACG184150.09.8954661
AGTACGG184900.09.7952952
ATAATAC19850.09.7858943
Produced by FastQC (version 0.11.5)