FastQCFastQC Report
Thu 28 Apr 2016
ERR1041489.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041489.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7191773
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT446950.6214740092603035No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT374120.5202055181663826No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT318660.4430896247698586No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183560.2552360871234395No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77440.10767859330376528No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74570.10368792229676882No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA144800.019.1515881
CTACGCT5850.013.28205230
GTATCAA210000.013.1878582
GACGCTA5900.012.54237326
CGCTATC6500.012.52307733
ATCTCGT6300.012.33333437
AACCGTA1851.0415042E-612.07
CGAGACG6750.011.78518523
TACGCTA6450.011.47286831
ACGCTAT6650.011.40601532
TCTATAC9000.010.8944443
GTATTAG19300.010.8316061
AACGCAG254100.010.7825667
GCTACGC7300.010.64383629
ACGAGAC8700.010.63218422
AACGGTA2801.268927E-810.57142918
CGCTACG7350.010.57142928
TTAACGG5900.010.34745835
TAGTACT8300.010.0301214
ATCAACG276500.010.0160934
Produced by FastQC (version 0.11.5)