FastQCFastQC Report
Thu 28 Apr 2016
ERR1041488.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041488.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10522896
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT492500.4680270526288581No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT468860.4455617541026729No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT353320.33576308271031097No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146700.13941029161554006No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA155950.023.3933961
GTATCAA240200.015.1496662
TTTAGCG5850.012.96581326
GTATTAG40250.012.6397521
TTAACGG15550.012.25401935
TAACGGC15500.011.93548336
AATACTG31250.011.89925
AACGCAG324400.011.200377
TAGGACC21250.011.143534
TAGACCG1851.3621528E-511.05
CTCTAAT26150.010.8240911
TAATACT42950.010.8114084
ATACTGG29900.010.7658866
TAGGGGT19750.010.6784814
TCTATAC14450.010.6262973
TTAGGAC26650.010.6210133
ATCAACG345350.010.5637764
TCTAATA31600.010.479432
TCAACGC350000.010.4181425
ATTAGAG22200.010.4166673
Produced by FastQC (version 0.11.5)