FastQCFastQC Report
Thu 28 Apr 2016
ERR1041487.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041487.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9639246
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT218170.2263351303618561No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT214590.2226211469237324No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT149440.15503287290312956No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA99950.017.8984491
GTATCAA142950.012.4368662
CGTATTA4400.012.19318215
TATACTG15100.012.0066235
CTAATAC24750.011.5858583
TAACCGG5900.011.28813622
GTATTAG24800.011.2641121
GGACCGT6950.011.1798566
CTAGTAC6150.011.1300813
TAATACT22200.011.04
TTAACGG8150.010.89570535
ATCCGTA4150.010.69879512
GTATTAA12550.010.6135451
GTATAAA16000.010.5218751
TAATACC11900.010.4159674
CTACACT16750.010.382094
TAGTACT11850.010.3037984
AACGCAG184000.010.2755445
GTACTAG5950.010.2605051
TAGACAG15950.010.2068965
Produced by FastQC (version 0.11.5)