FastQCFastQC Report
Thu 28 Apr 2016
ERR1041486.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041486.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7703303
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT683970.8878918562595811No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT610280.7922315920845903No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT454280.5897210586160249No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT344250.4468862252984207No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202310.26262760273093244No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA192870.2503731191671936No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146050.18959399623771778No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120020.1558032963262642No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118420.1537262652137661No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGT113720.14762498632080293No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101140.13129432919878656No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT79900.1037217411803742No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA138150.028.0010871
ATCTCGT20600.021.82281537
TCTCCGA22200.021.7511
ACGAGAC22150.021.71557622
AGACCGA22400.021.39062525
ACCGAGG23150.020.6177127
GACCGAG23450.020.59061826
CCCACGA22950.020.55555519
CGAGCCC23550.020.4246315
ATCTCCG23750.020.17473610
GAGACCG24050.020.07692524
CCGAGCC25700.019.07587414
GCCCACG25750.018.75145718
TCCGAGC27050.017.98706213
CACGAGA26650.017.97936221
TATACAC31500.017.8539683
GTATCAA217850.017.7314662
CGAGGCT28000.017.17857229
CCACGAG28850.016.54419520
TACACAT34800.015.7356335
Produced by FastQC (version 0.11.5)