FastQCFastQC Report
Thu 28 Apr 2016
ERR1041485.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041485.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7758518
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT338410.43617866195580135No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT295000.3802272547411761No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT237120.3056253784550091No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140440.18101395137576531No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111600.018.9475821
GTATCAA158050.013.2970592
CGCGAAA1851.3620249E-511.015
TTAACGG6100.010.91803335
AACGCAG189400.010.8616697
CTAGTAC5650.010.805313
GTATTAG19900.010.7839191
CTACACT12700.010.7795284
TTAGGAC13250.010.61132053
TAACGGC6350.010.48818936
ATCAACG202400.010.3376984
TAGGACC11900.010.2605044
CGCGATA1450.00249526510.20689714
TTCGCGA1450.00249526510.20689719
TCAACGC205500.010.1997575
CAACGCA206900.010.1039156
TATCAAC214350.010.0202933
TCTAGAC6400.09.8281253
GAGTACG110150.09.8084431
AGTACGG109900.09.6960882
Produced by FastQC (version 0.11.5)