FastQCFastQC Report
Thu 28 Apr 2016
ERR1041484.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041484.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10552032
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT293980.27860036815657874No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT264230.25040674630251314No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT204800.19408584052815608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98200.021.3823851
GTATCAA146950.014.2259272
TATACTG17550.012.8603995
TAGACAG16800.012.0029775
TTAACGG8950.011.57541935
AACGCAG196150.011.1763955
TAATACT30200.011.1490064
CTAATAC28900.011.0743953
TCTATAC15900.011.0534583
GTACTAG6200.011.0403231
TCTAATA22800.010.8728072
AATACTG26550.010.8700565
ATCAACG202300.010.6811662
GTATACG4850.010.6804121
CAACGCA205250.010.6267974
GTATTAG26500.010.61132051
TCAACGC205600.010.590713
GTATAGG19250.010.5714291
ATACTGT21350.010.3981286
TTAGGAC18200.010.2664833
Produced by FastQC (version 0.11.5)