FastQCFastQC Report
Thu 28 Apr 2016
ERR1041483.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041483.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10400674
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT151590.14575016965246676No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT145040.13945250086677075No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT112430.10809876359935904No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72150.019.3589741
GTATCAA106550.012.8831542
TATACTG18100.012.8784535
GTATTAG23850.012.3333341
TTAACGG8850.011.49717535
GTACTAG7150.011.3846161
TCTATAC13600.011.29044153
CTAATAC23300.011.115883
TAATACT23000.011.0195664
TAGACAG16400.010.9420735
ATACTGT23450.010.8081036
AACGCAG142800.010.649165
ATCAACG144500.010.408652
CTACGCT5000.010.364
TAGGACC17850.010.2605054
TCAACGC147000.010.2568033
CAACGCA147350.010.1696634
CTAGTAC7650.010.1568623
TAACGGC10050.010.12437836
GTCTTAT12800.010.11718751
Produced by FastQC (version 0.11.5)