FastQCFastQC Report
Thu 28 Apr 2016
ERR1041482.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041482.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9203914
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT236650.2571188735574887No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT214080.23259669744849854No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT160760.17466482194422936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81800.019.8569681
GACCGTA7300.014.44520626
TAGACCG1956.7484507E-1014.230775
GTATCAA120800.013.3696192
TATACTG15350.012.29315955
ACTAGAT9450.011.55026432
GTACTAG8750.011.41714330
GTATTAG20700.011.3502421
TCTATAC13750.010.8981823
TCTAGAC9400.010.8244693
TTAACGG7350.010.8231335
AACGCAG155950.010.6290485
ACCGTAC10350.010.54589427
TAATACT21950.010.5353084
CGTACTA9850.010.51776729
ATCTCGT10100.010.44059537
GTATTAA12450.010.2530121
AGTACTG19500.010.2461545
ATCAACG162450.010.1923682
TCAACGC163750.010.1566413
Produced by FastQC (version 0.11.5)