FastQCFastQC Report
Thu 28 Apr 2016
ERR1041481.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041481.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10503750
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT208440.1984434130667619No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT199040.18949422825181483No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT144400.13747471141259074No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94750.018.5488131
TTAACGG10550.014.20379235
GTATCAA138000.012.6416662
TAACGGC12250.012.23265336
TATACTG16200.011.7623455
GTATTAG27950.011.5831841
AACGCAG178750.011.1569235
TTTAACG14600.011.15068534
GGCGAAT8000.011.132
AACGGTA4400.010.93181818
TACACTG24100.010.9004155
AATACTG26600.010.8496255
TAACGCC4300.010.7558144
CTAATAC26850.010.7486033
ATTAGAG18800.010.7260643
CTACACT19200.010.5989584
GCGAATC8900.010.39325933
TAGACAG18550.010.3719675
ATCAACG190700.010.3704782
TCAACGC191500.010.3657963
Produced by FastQC (version 0.11.5)