FastQCFastQC Report
Thu 28 Apr 2016
ERR1041480.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041480.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7094897
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT312750.4408097820165676No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT309410.4361021731534651No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT225780.3182287212908094No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104530.14733124385033355No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83800.021.2816221
GTATCAA126750.014.0702172
TTAACGG5050.012.82178235
GTATTAG17300.012.1907521
AACGCAG166450.011.6701725
CTAGTAC4950.011.5858583
CGATCGT1451.9409055E-411.48275913
TCAACGC176900.010.9807813
ATCAACG176300.010.9656842
TAACGGC6100.010.91803336
CAACGCA178050.010.8890764
TAACGCC2053.5798475E-610.8292684
GTACGAC2604.8445145E-810.6730773
TATACTG11300.010.6415935
TCTATAC11350.010.5947133
GTCTATA6850.010.5328471
ATCTCGT7500.010.3637
ACGCAGA188700.010.35294156
TATCAAC184650.010.2694281
TAGGACC11200.010.2410714
Produced by FastQC (version 0.11.5)