FastQCFastQC Report
Thu 28 Apr 2016
ERR1041479.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041479.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7142868
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT155310.21743366950082235No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT145040.20305569135534912No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT109810.1537337663246752No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63450.020.234831
GTATCAA90200.014.2134142
CTAGTAC4650.013.5268833
TAGACAG11250.012.3333335
CTAATAC16250.011.9538463
TAATACT16200.011.7623454
GTATAGG12200.011.3729511
TAGACCG1502.6584938E-411.15
AATACTG16900.011.0562125
TTAACGG6400.010.98437535
TATATCG3053.765308E-1010.9180335
TATACTG11750.010.863835
AACGCAG117150.010.8489117
GTCTTAC7700.010.8116871
GTATTAG18200.010.7747261
CTACACT11650.010.4806874
ATCAACG122750.010.3991864
TCAACGC123500.010.3809715
TAGTACG5350.010.3738324
CAACGCA124000.010.2794356
Produced by FastQC (version 0.11.5)