FastQCFastQC Report
Thu 28 Apr 2016
ERR1041478.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041478.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5221692
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT111660.2138387327326085No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT109710.21010431101642918No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT80850.15483486961697474No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45300.019.561811
GTATCAA66100.013.2942512
TATACTG8800.013.0340915
CTCTAAT9050.011.6519341
TAGGACC7500.011.5933344
TAGACTG6450.011.4728685
AATACTG13350.011.3632965
AACGCAG89600.011.3560275
TAATACT14900.011.2986584
GTCTATA3650.011.1506851
TAGACTA4550.010.9780235
TAGACCG1350.001376387910.9629635
GTGTTAC6450.010.8992241
ATCAACG94200.010.8407642
TCAACGC95150.010.8102993
CAACGCA95250.010.7018374
CGATCGC4150.010.69879519
TCTAATA10600.010.6462272
TTAACGG5050.010.62376335
GTATAGG10300.010.5970881
Produced by FastQC (version 0.11.5)