FastQCFastQC Report
Thu 28 Apr 2016
ERR1041477.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041477.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6250335
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT144190.23069163492836783No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT137000.21918825150971907No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT101220.16194331983805668No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58350.018.5792621
GTATCAA82150.013.0389532
TTAACGG5350.011.41121535
TTATACG1951.9641047E-611.38461635
GTACTAG2952.1100277E-1011.2881361
GTCTATA6100.011.2213121
TAGACCG1502.6583934E-411.15
CGCGAAA1502.6583934E-411.115
TAAGACT12550.011.0557784
TAGACAG10650.010.9436625
CCTACAC11000.010.7636363
TAACCGG3801.8189894E-1210.71052622
TATACTG10100.010.6237635
GTATTAG16400.010.603661
TATACCG2301.2443979E-610.4565225
TCTAGAC7000.010.3071433
TAATACC7900.010.3037984
AACGCAG110150.010.211535
TCAACGC111500.010.1210763
ATCAACG110600.010.1198012
Produced by FastQC (version 0.11.5)