FastQCFastQC Report
Thu 28 Apr 2016
ERR1041476.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041476.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10434611
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT724330.6941609993894358No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT524840.5029799385909067No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT396310.37980332951559No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115320.11051681754116181No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA128500.027.3252931
GTATCAA304000.021.2019731
ATCAACG453250.014.0734693
TATCAAC456500.014.0178542
TCAACGC458350.013.9007314
CAACGCA462700.013.7740445
AACGCAG465150.013.7174026
ACGCAGA493350.012.9408147
CGCAGAG500800.012.7372218
GTATTAG32400.012.7330251
TTAACGG12250.012.68571535
GCGAACT14700.012.33333328
CAGAGTA518700.012.28340110
AGAGTAC518900.012.26440511
GCAGAGT524350.012.1510449
ACGAACG5650.012.11504529
CGAACTA15000.012.08666629
TAACGGC13050.011.76628336
GTATTAT23200.011.3232761
CGGATCG3700.011.037
Produced by FastQC (version 0.11.5)