FastQCFastQC Report
Thu 28 Apr 2016
ERR1041475.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041475.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10336172
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT696790.6741277138190038No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT590280.5710818279726769No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT435230.4210746492995666No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124160.12012184007773866No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA141750.026.976721
GTATCAA311000.019.6004831
CGAACGC4950.013.82828130
CGAACTA11650.013.02145929
TATCAAC471950.012.8808142
ATCAACG474750.012.7230113
TCAACGC480950.012.5474594
AACGCAG484450.012.4797196
GCGAACT11900.012.43697528
CCGTTAA13250.012.42641516
CAACGCA486850.012.3953985
GTCGCCC15050.012.29235837
GTATTAG35750.012.2643361
ACGAACG5450.012.22018329
TTAACGG12900.011.90310135
GCTCGGA16050.011.757009511
ACGCAGA515750.011.7331087
TAACGGC13450.011.6914536
GCCGTTA14350.011.60278715
CGCAGAG523650.011.5631638
Produced by FastQC (version 0.11.5)