FastQCFastQC Report
Thu 28 Apr 2016
ERR1041474.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041474.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14429840
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1008390.6988227173690076No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT694400.48122501704800613No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT497030.3444459536626879No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148310.10278007240551523No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA192500.024.0932461
GTATCAA449950.020.5989551
TTAACGG21100.015.95734735
TAACGGC22400.014.86607136
TATCAAC637300.014.3924362
AACGCAG637550.014.3171526
ATCAACG639350.014.30577853
TCAACGC647000.014.1080374
CAACGCA653050.013.980175
TTTAACG24700.013.63157834
AACGGCC25000.013.61637
ACGCAGA673050.013.57848557
CGCAGAG680050.013.4387198
CAGAGTA704950.012.94829510
AGAGTAC705200.012.91222311
GCAGAGT710400.012.843759
GTATTAG57850.012.6318061
CGAACTA21500.012.21860429
GCGAACT22000.012.02528
CCGTTAA17600.011.77272716
Produced by FastQC (version 0.11.5)