FastQCFastQC Report
Thu 28 Apr 2016
ERR1041473.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041473.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15226271
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1215990.7986131338395329No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT829410.544722998822233No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT624490.4101398168993577No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193120.12683341837275852No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA280050.020.683271
GTATCAA589450.019.0288411
GTATTAG54100.014.1913121
TATCAAC817250.013.6681562
AACGCAG820700.013.5430746
ATCAACG821500.013.5388933
TTAACGG19100.013.4633535
TCAACGC831200.013.3653154
CAACGCA836500.013.2872685
TAACGGC19550.013.24808136
ACGCAGA871050.012.7793477
CGCAGAG877750.012.6712628
AGAGTAC899950.012.34635311
CAGAGTA908800.012.24851510
GCAGAGT919350.012.1059459
CCGTTAA14500.011.86551816
AACGGCC22200.011.83333237
CGAACTA18500.011.829
GCCGTTA14750.011.66440715
ATCGTAC9400.011.21808425
Produced by FastQC (version 0.11.5)