FastQCFastQC Report
Thu 28 Apr 2016
ERR1041472.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041472.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9328133
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT612050.6561334406359772No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT599820.6430225641079517No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT415700.4456411588471133No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT209410.22449293979834978No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135980.14577407933613296No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG118460.12699218589614877No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA246850.015.3860651
GTATCAA412650.014.4628621
GCTCGGA16650.011.88888911
AGGTCGC16200.011.53395135
GTCGCCC15450.011.25566437
CGAACTA11600.011.00431129
GTATTAG29650.010.981451
CCGTTAA9100.010.97802216
TGCTCGG19800.010.83838410
GCGAACT11850.010.77215228
GTATTAA22750.010.6527471
GCTCCGA17550.010.64672429
CTATCGC6000.010.4833349
TGTACTG37200.010.3938175
TTGTACG9800.010.3826539
TTAACGG11300.010.31415935
TATACTG22800.010.3048245
ATAATAC32750.010.2809163
TATCAAC579450.010.2804382
TCACGTA12050.010.1327825
Produced by FastQC (version 0.11.5)