FastQCFastQC Report
Thu 28 Apr 2016
ERR1041471.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041471.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14517406
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1139450.7848853989479938No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT791070.5449113980831011No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT607020.418132550677442No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194600.134045985901338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA272350.021.172941
GTATCAA575800.018.9915751
GTATTAG47450.014.0358271
TATCAAC803950.013.5306922
ATCAACG811850.013.3625673
AACGCAG817550.013.3056096
TCAACGC821600.013.1972374
CAACGCA826700.013.1337245
TTAACGG13500.013.01851835
ACGCAGA867550.012.549427
TAACGGC13850.012.42238236
CGCAGAG876050.012.4213248
AGAGTAC891750.012.16529411
CAGAGTA905700.012.00242910
GCAGAGT912800.011.9110989
ATAACGC7350.011.326533
CGAACGC7550.011.02648930
TAGGACC20400.010.9730394
GAGTACT413250.010.95002912
CGAACTA17250.010.9391329
Produced by FastQC (version 0.11.5)