FastQCFastQC Report
Thu 28 Apr 2016
ERR1041469.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041469.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16049304
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT979360.6102196082770941No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT625410.38968044969426713No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT520500.3243131290927008No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163080.10161188298258915No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA193300.025.1994321
GTATCAA439650.020.1095181
CGAACTA19250.015.18441629
GCGAACT19800.015.0429328
ATCAACG603450.014.5283783
GTATTAG55100.014.4709631
TATCAAC611750.014.4098892
AACGCAG608600.014.4084786
TCAACGC613050.014.2978544
TTAACGG22100.014.23076935
CAACGCA616550.014.219695
TAACGGC23250.013.60645136
ACGCAGA649150.013.5112847
CGCAGAG658700.013.3210128
AGAGTAC680600.012.86519311
CAGAGTA682950.012.83175910
GCAGAGT688750.012.7237029
AACGGCC26800.012.08022537
TTAGACT20400.011.6985294
TCTATAC34300.011.596213
Produced by FastQC (version 0.11.5)