FastQCFastQC Report
Thu 28 Apr 2016
ERR1041468.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041468.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13959045
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT958000.6862933674903978No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT649610.46536851195765905No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT463770.3322361952411501No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA141950.028.163791
GTATCAA375300.022.5716761
TATCAAC553650.015.1969662
ATCAACG554600.015.1042193
TCAACGC558100.014.9862924
CAACGCA563950.014.8406785
AACGCAG565600.014.826826
ACGCAGA599600.013.9768177
CGCAGAG605950.013.830358
TTAACGG14250.013.242105535
CAGAGTA632700.013.23391810
AGAGTAC633300.013.19508911
GCAGAGT643050.013.0237939
TAACGGC14650.012.75426636
GTATTAA30950.012.4927291
GTATTAG40850.012.3182371
ACGAACG5150.012.21359229
CGAACTA15600.011.97756529
CGAACGC5800.011.80172430
GCGAACT16050.011.75700928
Produced by FastQC (version 0.11.5)