FastQCFastQC Report
Thu 28 Apr 2016
ERR1041467.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041467.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6507272
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT449270.6904122034548426No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT380710.5850531528419283No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT266390.4093727755655519No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG253720.38990225089715014No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG163110.25065803304364714No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG149500.22974297063346974No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT148110.22760689886637595No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133730.2055085449017653No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT118610.1822730016510759No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAG110660.17005590053712216No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG103940.15972899242570465No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTA97460.1497709024611235No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT80470.12366165114966762No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG70190.10786394052684442No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAA70190.10786394052684442No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTG70110.10774100114456565No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGT67910.1043601681318992No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTG66890.10279269100784477No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGC3000.014.83
TTAACGG4150.013.37349435
CTAGTAC6300.013.2142853
ACGTTTA4350.013.183907526
TAACGCC3650.013.1780824
GTATACG7300.012.9246581
TCTAACG3150.012.9206342
GTCTTAC5300.012.5660381
GTACTAG5050.012.4554441
TATACGG7300.012.4178092
TATACTG9450.012.3333335
TAATACT14000.012.2892864
GTATTAG15200.012.0493411
ATAACGC5750.011.9043483
TAGGGGT7850.011.783444
ACCGTCC5200.011.7403858
ATCTCGT6950.011.7122337
TCTAGAC5850.011.7008553
GGGGTTA9200.011.6630446
GGGTTAG8150.011.3496937
Produced by FastQC (version 0.11.5)