FastQCFastQC Report
Thu 28 Apr 2016
ERR1041466.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041466.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6820350
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT236760.3471376102399437No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT230020.3372554194432837No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT160900.23591164676299603No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72180.10583034594998791No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA129000.014.1403081
TCGCTAA1602.7003462E-612.7187514
CTAGTAC5400.012.3333343
TAGACAG9950.011.8994975
GTACTAG4900.011.70408251
GTAAGAC7750.011.6967743
TAGTACT10450.011.5071764
TATACTG11600.011.3232765
CTAGACA8550.011.2514624
GTGTAAG12450.011.1445781
TATACCG3850.011.0519485
TTAGTCG1553.6010993E-410.74193528
CGCGAAA2604.8443326E-810.67307715
CTAACGC3156.6029315E-1010.5714283
TTAACGG2801.2687451E-810.57142835
TCTAGAC6150.010.5284553
TGCGACG2656.406117E-810.47169822
TAAGACT8850.010.4519774
GTCTAAT6550.010.4503811
GTATCAA174650.010.4337242
Produced by FastQC (version 0.11.5)