FastQCFastQC Report
Thu 28 Apr 2016
ERR1041465.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041465.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7241566
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT177540.2451679650506534No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT169000.23337493575284682No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT119370.16484003598116762No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA80500.017.7416131
GTATCAA112050.012.6800542
TATACTG11400.012.6578945
TAGACAG12750.012.0431385
TCTAGAC7150.011.9020983
GTATAGA10350.011.260871
AACCGTA2002.6625403E-611.17
TAGACTA7500.011.0999995
GTATTAG19000.010.9052631
CTAGACA10100.010.8069314
CTAGACG1901.8297276E-510.7105264
TCTATAC10150.010.5714283
TAATACT19250.010.4753244
TAGACCG1952.437756E-510.4358985
TTAGACT7300.010.3904114
TACGTAG3452.9649527E-1010.18840614
CTAACGC2003.222317E-510.1753
AACGCAG142800.010.1568635
AATACTG18700.010.090915
CGCGAAA2751.10161636E-710.09090915
Produced by FastQC (version 0.11.5)