FastQCFastQC Report
Thu 28 Apr 2016
ERR1041464.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041464.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9968777
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT578230.5800410622085337No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT487500.4890268886544457No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT416800.41810545064856No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219210.21989658310141758No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA182800.019.1375831
GTATCAA266850.013.1028672
TTAACGG6600.011.77272835
TATACTG17600.010.8267045
AACGCAG322450.010.6656857
TAATACT25500.010.447064
TCTATAC14600.010.26373
GTCTAGA9950.010.22613051
AACGGTA5850.010.11965818
ATCAACG348750.010.0045874
GTATTAG26450.09.8620041
TATCAAC359550.09.8481443
TAACGGC7900.09.83544336
GTATAGG17550.09.8034191
TCAACGC356000.09.7904495
CAACGCA360900.09.6677756
GTACTAG6700.09.6641791
TAAGTCG2301.410639E-59.6521745
CGCGAAA2301.410639E-59.65217415
CTAATAC25500.09.649023
Produced by FastQC (version 0.11.5)