FastQCFastQC Report
Thu 28 Apr 2016
ERR1041463.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041463.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8998091
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT451260.5015063750744463No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT433410.4816688339782294No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT300940.3344487180669767No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120960.1344285137814232No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA172950.019.1150631
GTATCAA251800.013.1145752
TCGCTAA2002.6627768E-611.114
GTATTAG23500.010.94255351
AACGCAG329700.010.8014865
TAGACTG10150.010.7536955
ATCGATA4850.010.68041223
TAATACT25200.010.5714284
GCTATAC7200.010.5347223
TATACTG13700.010.5328465
TAGCGCG6950.010.3812954
ATCCGTA3402.2737368E-1010.33823512
TAGACCG1801.2478865E-410.2777795
GTACTAG5600.010.2410721
TACGTCG2208.319481E-610.0909095
TACTCCG13600.010.0661765
TTAGGAC15650.010.0479233
TACGACG2952.8554496E-810.0338985
TATAGAC9600.010.0208333
CTACACT18400.09.9538054
Produced by FastQC (version 0.11.5)