FastQCFastQC Report
Thu 28 Apr 2016
ERR1041462.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041462.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7399990
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT311910.4215005695953643No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT290770.39293296342292355No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT218370.29509499337161266No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94110.1271758475349291No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA127050.016.7599371
TTAACGG6100.013.34426235
GTATTAG19600.012.0816331
GTATCAA178900.011.8921182
TATATCG3900.011.85897355
TCTAACG2757.876224E-1011.4363632
CGATCGC6350.011.07086719
CTACACT14450.011.0103814
TAGGACC9150.010.9180344
TACACTG15100.010.7814565
TTTAGCG5150.010.77669926
GTACTAA4300.010.7558141
TAACGGC7500.010.606667536
TAATACT22350.010.5950784
AACGCAG210650.010.5651565
AATACTG17850.010.4677875
TATACTG9750.010.4358985
GTTTAGG16150.010.4241491
TATAACG3208.6765795E-1010.406252
CTAACGC2503.23902E-710.3600013
Produced by FastQC (version 0.11.5)