FastQCFastQC Report
Thu 28 Apr 2016
ERR1041461.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041461.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8568019
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT194300.2267735400680134No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT183520.2141918686221401No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT134130.15654727189563888No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA101550.015.9768581
TATACTG14800.012.255
TTAACGG6300.012.03968335
TAAGACT14350.011.7317084
GTACTAG6400.011.56251
TAATACT17050.011.5014674
TAGACAG16600.011.1445785
GTATCAA145550.011.0961872
ATAATAC11250.011.0177783
CTAGTAC6750.010.9629633
CGCGAAA2401.8467654E-710.79166715
GTATTAG19800.010.7449491
GTATACG4250.010.4470581
TAACGGC7450.010.4295336
TAGTACT11600.010.3663794
GCGTAGA4650.010.3440861
TATACCG4150.010.2530125
TCTATAC11250.010.1955563
TAGGACC12950.010.1428584
CTAATAC20700.010.0990343
Produced by FastQC (version 0.11.5)