FastQCFastQC Report
Thu 28 Apr 2016
ERR1041460.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041460.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7124204
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT189850.26648591196995486No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT176430.2476487197727634No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT134870.18931237791618544No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96350.017.8951741
GTATCAA138050.012.4762772
CGCGAAA1757.3470383E-611.628571515
TAACCGG4550.011.38461522
GTATTAG19700.011.3629451
TATACTG10600.011.1698115
TAGACCG1502.6584917E-411.15
AATACTG14750.011.0372895
TAGCACT10900.010.8623854
TAATACT17800.010.808994
CTAGTAC4650.010.7419363
TCTATAC7950.010.7044023
TTAACGG5550.010.66666735
CTAATAC19400.010.5850523
CTAGGAC12400.010.4435483
AACGCAG173250.010.4326125
AGACCGT7500.010.1133346
GTACTAG4400.010.0909091
TAGACAG11100.010.05
GTAGCAC6850.09.9927023
Produced by FastQC (version 0.11.5)