FastQCFastQC Report
Thu 28 Apr 2016
ERR1041459.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041459.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12047491
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT880220.7306251567235036No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT817850.6788550412695888No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT620220.5148125862887136No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT291130.2416519755026171No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA294800.018.4058691
TATACTG19900.013.293975
GTATCAA418350.013.0408752
ATCTCGT11750.012.59574437
GTATTAG27250.011.6091751
GGACCGT6100.011.2213126
AACGCAG500450.010.9495455
TAATACG6000.010.7916674
AACGGTA6400.010.69531318
TAATACT28000.010.5714294
TTAACGG7400.010.535
CTAGTAC9550.010.4607323
CCCACGA13800.010.45652219
TCTATAC17050.010.4164233
CTAATAC28550.010.2381783
TAGTACT15500.010.0258074
GTCTTAC12500.09.9161
TCTAACG3554.984031E-109.9014082
TAGACTG13900.09.8489215
TAACGCC5450.09.8440374
Produced by FastQC (version 0.11.5)