FastQCFastQC Report
Thu 28 Apr 2016
ERR1041458.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041458.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7547850
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT169540.22462025609941905No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT155810.20642964552819676No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT110650.14659803785183861No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87800.017.8468111
GTACTAG5500.013.790911
GTATCAA125400.012.5103672
TTAGACT7850.012.2547774
ATCTCGT8000.012.02537
TACACTG16400.011.7317085
CTACACT15950.011.4827594
AATCTCG7950.011.40251536
GTATTAG19050.011.2650921
TAGACTG10850.011.2534565
ACGAGAC8950.011.1620122
TATACAC13800.011.1268113
CTAGTAC6450.010.8992243
TTTAGAC8100.010.7345683
TTAACGG6400.010.69531335
AACGCAG158100.010.5781155
CGATCGT1400.001863973810.57142913
TAGACAG12850.010.3657595
TCGCTAA3402.2737368E-1010.33823514
TAATCGG5550.010.33333316
Produced by FastQC (version 0.11.5)