FastQCFastQC Report
Thu 28 Apr 2016
ERR1041457.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041457.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5377827
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT254290.47284897784923163No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT243240.45230164525560235No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT170380.31681941423552673No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83620.1554903123510667No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTA6650.016.6917326
ACTAGAT7900.014.51898732
GGTATCA132350.014.3135621
CGTACTA7650.014.26797429
ACGAGAC7950.014.19496922
TATACAC10950.014.0228313
CTAGTAC4600.013.6739133
ATCTCGT7750.013.60645237
GTACTAG8250.013.23030230
ACCGTAC8850.012.96045327
TATACTG8150.012.7116575
GTATTAG11800.012.6991531
CCCACGA9050.012.46961319
ATAATAC6800.012.2426473
GGACCGT3051.8189894E-1212.1311476
ATCTCCG9800.012.08163310
GAGACCG9250.011.824
CTAGATC9150.011.72677533
TCTCCGA10000.011.28511
TACTAGA9900.011.21212131
Produced by FastQC (version 0.11.5)