FastQCFastQC Report
Thu 28 Apr 2016
ERR1041456.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041456.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5805250
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT98190.16914000258386805No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT89960.1549631798802808No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT65950.11360406528573275No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64850.015.4903621
GTACTAG4700.015.3510631
GCGAATC6250.013.61633
TCTAGAC6300.013.2142853
GACTAAG6600.012.8939426
CTAGTAC5400.012.3333343
ATCTCGT7050.012.33333337
TATACAC10650.011.9859153
TTAACGG4900.011.704082535
GGCGAAT7450.011.67114132
TAAGGCG7800.011.38461629
TGCGACG3104.1836756E-1111.3387122
GTATCAA87950.011.3166572
ACGAGAC8550.011.25146222
GTCTTAG12250.011.175511
TAGTACT8450.011.1656814
TATATCG2502.7202987E-811.0999995
CGATCGT2053.5794837E-610.829267513
GGACTGT12500.010.6566
AACGGTA3301.3096724E-1010.65151618
Produced by FastQC (version 0.11.5)