FastQCFastQC Report
Thu 28 Apr 2016
ERR1041454.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041454.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3831665
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT136930.357364226778698No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT108020.28191399822270474No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT66500.17355379449925815No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43240.11284911389696124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32350.025.7913461
GTATCAA45600.018.175442
GTATACG1857.39783E-813.01
TTAGACT4200.012.7738094
TATCAAC69700.012.4748933
TCAACGC67650.012.3606815
ATCAACG67900.012.3424164
CAACGCA68100.012.1431726
AACGCAG69450.011.9337657
TATACTG6900.011.7971015
TACCGTC2052.8160503E-711.7317087
TAATACG2301.02512786E-711.2608694
ATAATAC5550.011.03
TCACGTA3301.3096724E-1010.65151525
TAATACT9050.010.6298344
ACGCAGA79550.010.441868
TATTCCG1604.826448E-410.406255
TTACACT8200.010.378054
ATACTGT8700.010.2068976
TAGACTG4550.010.1648365
Produced by FastQC (version 0.11.5)