FastQCFastQC Report
Thu 28 Apr 2016
ERR1041453.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041453.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7078180
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT226460.3199410017829442No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT208840.29504759698114486No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT158300.22364506130106893No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95150.021.0761951
CGCGAAA2059.276846E-1114.43902415
GTATCAA144050.013.9600832
TTAACGG4350.011.90804735
GCGTAGA2351.09084795E-811.808511
TCTAACG2552.9376679E-911.6078432
TCTATAC8200.011.5060973
GTATTAG18950.011.3245381
CTAGTAC6050.011.314053
ATACCGA2952.1100277E-1011.2881366
CTACACT14350.010.8292684
TAGACAG11350.010.75770955
AACGCAG192850.010.72491555
TATATCG3301.3096724E-1010.6515165
TAGTACT9400.010.6276594
TAATACT15200.010.4671064
AACGGTA3554.5474735E-1110.42253618
GTCTTAC6400.010.406251
TCTGTCG9900.010.2777788
CTAGACA9550.010.2670154
Produced by FastQC (version 0.11.5)