FastQCFastQC Report
Thu 28 Apr 2016
ERR1041451.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041451.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9485170
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT331640.34964054413363177No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT318740.33604036617161315No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT230800.24332721501037938No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125020.13180575572182682No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT116280.12259137158321885No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT9400.014.56382837
TTAACGG19400.013.73195835
TAATACT40450.012.943144
TATACTG12450.012.7791165
AATACTG34750.012.7769795
TAGACCG1451.374348E-512.758625
TAACGGC21100.012.62559236
GGTAAAC25500.012.62352935
GGTATCA209400.012.4216811
ATACTGG32050.012.3525736
GTATTAG38150.012.3171691
TCTAATA33450.011.9461882
ATTAGAG28150.011.8952053
TACTGGT30900.011.79449757
TTGGTAA28900.011.58650633
ACCGCCT24950.011.56713412
TCGGACG6400.011.562513
TCACGTA27050.011.48983325
CGAACTA23900.011.3786624
AGACCGA12400.011.18951625
Produced by FastQC (version 0.11.5)