FastQCFastQC Report
Thu 28 Apr 2016
ERR1041450.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041450.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12167531
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1276561.0491528642910382No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1146500.9422618278104243No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT856880.7042349018876549No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT394750.32442900700232447No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138540.11386040438277904No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT136550.11222490413215302No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA451900.016.3998661
TAATACT32450.014.537754
TTAACGG14300.014.48951135
GGACCGT12700.013.1102366
TAACGGC16400.012.859755536
AATACTG31150.012.8282515
TCTAATA27200.012.1746322
ATTAGAG21450.012.0745923
GTATCAA623450.011.943622
TCTATAC12100.011.9256193
TAGGACC21500.011.8744194
GTATTAG37400.011.6243311
TATTAGA27950.011.3846162
AACGCAG756900.011.1430185
TTAGGAC23000.011.13
TTTAACG19200.011.08072934
CTAATAC44900.010.9599113
TCGGTAA7100.010.94366223
ATAATAC14800.010.753
CTCTAAT22050.010.6553281
Produced by FastQC (version 0.11.5)