FastQCFastQC Report
Thu 28 Apr 2016
ERR1041449.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041449.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8702358
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT294400.33829911387235506No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT262190.3012861571541874No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT198390.2279726942973387No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127410.14640859408449985No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG100540.11553190526062017No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82750.023.5190321
TCGCTAA2150.017.20930314
GTATCAA129800.015.0508472
CGCGTAT750.00410728814.812
AATACTG32150.013.9828935
TTAACGG18500.013.535
CTCTAAT29300.013.4488051
ATACTGG30600.013.3006536
TACGACG2800.013.2142865
TAATACT43900.013.1902064
GGTAAAC25550.013.178081535
TACTGGT29050.012.9277117
TCTAATA33700.012.7908012
ATTAGAG24300.012.7901243
ACCGCCT21550.012.53364212
TAACGGC20050.012.4563636
GTATTAG39650.012.31778051
GGCACCG22950.011.7690639
GATGCTA28950.011.758203514
TCTATAC10700.011.75700953
Produced by FastQC (version 0.11.5)